Materials and methods rats were immunized with fca by intradermal injection into the right hind metatarsal footpad, and were orally treated daily with taraxasterol at 2, 4 and 8 mgkg from day 2. Fifteen adult male wistar rats were studied in an adjuvantinduced arthritis model by the injection of complete freunds adjuvant in the right hind limb and right. Pharmacokinetics of morphine in rats with adjuvantinduced. Suppression of adjuvantinduced arthritis in the rat by gold sodium thiomalate. Adjuvantinduced arthritis in the temporomandibular joint. An acute toxicity study of butanol fraction was conducted, accompanied by a study of its antiarthritic activity. Journal of environmental pathology, toxicology and oncology. All of the rats were housed in separate cages with free access to water and food.
Inhibition of induced inflammation in the rat knee joint. Evaluation of antiinflammatory potential of the new. Here, we showed that in aa rats, ugt activity in liver. Kohashi o, kuwata j, umehara k, uemura f, takahashi t, ozawa a. Therefore, as a part of our ongoing screening program to evaluate the antiinflammatory potentials of natural compounds, the present study was designed to confirm the in vivo antiarthritic effect and clarify the potential mechanism of taraxasterol on adjuvantinduced arthritis in. We started the treatment after 4 days of disease induction early treatment group and after 11. Susceptibility to adjuvantinduced arthritis among germfree, speci. Ethanol extracts of ayurvedic formulations vatari guggulu, pathyadya churna and shatpuspadya churna were administered to the rats by oral gavages in different doses. Jan 26, 2001 to verify our working hypothesis concerning the appropriateness of immunomodulatory therapy with ribomunyl, the adjuvant arthritis model in rats was chosen. To test these indications, we used adjuvant arthritis aa in rats, a classic experimental model of polyarthritis. Suppression of adjuvantinduced arthritis in the rat by gold.
Evaluation of complete freunds adjuvantinduced arthritis in a wistar rat model article pdf available in zeitschrift fur rheumatologie 724 may 20 with 3,443 reads how we measure reads. Losartan, prednisolone, and complete freunds adjuvant cfa were. Arthritis was induced by administration of complete freunds adjuvant cfa into the subplantar surface of left hind paw of the animals. Pdf antirheumatoid activity of aqueous extract of piper. Antiinflammatory and analgesic effects of yaotuitong. The equipment used included a sartorius2452 analytical balance sartorius ag, gottingen, germany and a shimadzu510 fluorescence photometer shimadzu,tokyo, japan modelpreparation the model rats4 were depilated dorsally 2 days prior to the operation. Inflammatory mediators, including cytokines and free radicals play a vital role in many diseases, putting inflammation and oxidative stress at the. Sueceptibility to adjuvantinduced arthritis among germfree, specificpathogen free, and conventional rats. The aim of this study was to evaluate the progression of arthritis in a complete freunds adjuvant cfainduced wistar rat model and to monitor inflammatory arthritis activity using thermal imaging compared with histopathology. Adjuvant arthritis, induced by injections of freunds complete adjuvant into the footpads of some rat strains, has been recognized as a useful animal model for. Antirheumatoid activity of ethanolic extract of sesamum. Since ra is characterized by polyclonal bcell activation that gives rise to bcell expansion and the production of autoantibodies, btk is. A study of interleukin 6 il6 and tumor necrosis factor.
Due to alterations in the metabolic activities of arthritic rats, there may. May 05, 2015 arthritis was induced by a single intradermal dose 0. Pia is induced by intradermal injection of pristane into the tail base. Arthritis was induced by a single subplenter injection of 0. Disorders of the temporomandibular joint tmj frequently present an inflammatory component 2. Animals had free access to standard pellet diet and water ad libitum. Suppression of complete freunds adjuvantinduced adjuvant. We further examined the immunological mechanism of the combination of them by testing the rats secretion of il12. Adjuvant arthritis, induced by injections of freund s complete adjuvant into the footpads of some rat strains, has been recognized as a useful animal model for many years. The results indicate that rg1 has therapeutic effects on aia rats, and the mechanism might be. Ginsenoside rg1 attenuates adjuvantinduced arthritis in rats via. Development of an animal model of rheumatoid arthritisinterstitial lung disease raild and improved knowledge of the pathogenesis of raild may facilitate earlier diagnosis and the development of more effective targeted therapies.
Antiarthritic activity of methanol extract of cyathocline purpurea whole plant in freunds complete adjuvantinduced arthritis in rats gopal v. Low dose methotrexate is the cornerstone for the treatment of rheumatoid arthritis. The adjuvant induced arthritis aia model, one of the longest established animal models for testing arthritis treatments, is a reliable model with less welldefined mechanism than cia or pia. Antiarthritic effect of fty720, a novel immunosuppressant, was compared with those of immunosuppressants cyclosporin a and tacrolimus in adjuvant induced arthritis in rats. Adjuvant arthritis aia in rat antigen induced knee arthritis mouse rat adjuvant arthritis aia is an experimental model of polyarthritis that has been widely used for preclinical testing of numerous antiarthritic agents, which are either under preclinical or clinical investigation, or are currently used as therapeutics in this disease 24. In 1956, pearson found that rats immunized with complete freunds adjuvant cfa, mineral oil containing heatkilled mycobacterium tuberculosis mt particles, developed polyarthritis. Arthritis was induced by administration of complete freund s adjuvant cfa into the subplantar surface of left hind paw of the animals. Animals were divided into five groups containing six animals in each group one normal, one control, one standard and two test groups. The purpose of the present study was to observe the effect of nghjty on arthritis and to determine its. Chemokine receptor expression in rat adjuvantinduced. Pdf evaluation of complete freunds adjuvantinduced. Impact of oxidative stress on inflammation in rheumatoid and.
Da rats are extremely susceptible to aia, collageninduced arthritis cia as well as oil inducedarthritis oia, which is triggered by the injection of incomplete freunds adjuvant or pristine alone. The lesions in adjuvant arthritis are somewhat less analogous to those seen in human ra than are the lesions of type ii. Antiinflammatory and antiarthritic effects of taraxasterol. Protocol for induction of adjuvantinduced arthritis aia.
Within some days animals develop arthritic symptoms in the limbs incidence. Evidence is available to suggest a neurally mediated component to the inflammatory process. The aim of the present study was to investigate the therapeutic efficacy of synthetic peptides hpr1, hpr2 and hpr3, derived from the sequence of 65kd mycobacterial heat shock protein hsp, in the treatment of ra using adjuvant induced arthritis aa animal model. Since this discovery, aia has been widely used as a model for rheumatoid arthritis ra. Antiarthritic activity of methanol extract of cyathocline.
Adjuvant composition, route of injection, and removal of depot site. The first page of the pdf of this article appears above. All the rats, except those in the normal control group, were injected intradermally with 0. Ghjty is composed of 18 medicinal herbs, of which five primary herbs were selected and named new ganghwaljetongyeum nghjty. Freunds complete adjuvant fca induced arthritis in rats. A few experimental studies have evaluated only the immediate events of the inflammatory process in the tmj 8 special features of the tmj, such as its.
We investigated whether rats with antigen induced arthritis aia exhibit peripheral changes in skin innervation remote from the inflamed joint. Evaluation of complete freunds adjuvantinduced arthritis in. Arthritic rats were allocated into three groups n 10, the first group arthritis control, received 1% of tween 80, the second and the third groups received prednisolone 10 mgkgday. The male wistar rats, with rheumatoid arthritis induced by subcutaneous injection of 0. The experimental protocol was approved by the local ethical committee. The administration of extract reported significant reduction in paw swelling on 4 th, 8 th, 14 th and 21 st day after subplenter administration of complete freunds adjuvant. Arthritis was induced by injection of complete freunds adjuvant cfa in rats. Induction of adjuvant arthritis in mice knight 1992. Rats were housed in well ventilated opaque propylene cages with free access to. Rats were killed at various times postadjuvant injection, and joints harvested. After confirming arthritis induction using complete freunds adjuvant cfa in rats, nghjty 62. Suppression of adjuvantinduced arthritis in the rat by. After several minutes, it is metabolized by glucuronidation via a udpglucuronosyltransferase ugt. Arthritis was induced in rats by subcutaneous injection of 0.
Proinflammatory cytokines like interleukin1 and tumor necrosis factoralpha tnf. Following drugs and their combinations were orally administered to animals in a longterm prophylactic course. Pristane induced arthritis pia in the rat is a well characterized animal model for rheumatoid arthritis ra in man. Establishment of a rat adjuvant arthritisinterstitial lung. Adjuvant arthritis aa was induced in 40 rats by the subplantar injection of 0. The animals were maintained with free access to standard diet and tap water. Adjuvantinduced arthritis, its evaluation, and treatment the animals were randomly divided into 5 groups. Effect of losartan in complete freunds adjuvant induced arthritis in rats.
Antiinflammatory effect of herbal preparations on adjuvant. With the adjuvant arthritis aa rat models, we observed the protective effects of oral sb, gc and arthrigia in different concentrations on aa in rats. Pdf suppression of complete freunds adjuvantinduced. The rat adjuvant arthritis is an experimental model of. Effect of arthrigia on adjuvant arthritis rats il12. The energy cost of adjuvantinduced arthritis in rats. In the present study, we determined the in vivo antiarthritic effect of taraxasterol on arthritis induced by freund s complete adjuvant fca in rats. Aa is most easily induced with mycobacteria suspended in oil, although in some strains of rats it can be induced with oily adjuvants in the absence of mycobacteria. Arthritis was induced by injecting into the right hindpaw 100. The spread of pain is usually attributed to mechanisms in the central nervous system. Antigeninduced arthritis in rats is associated with.
Strains of rats have a varying genetic susceptibility to aa, whereas mice generally are not susceptible. Antiarthritic and hepatoprotective effect of derinat on. Effect of losartan in complete freunds adjuvant induced arthritis. Adjuvant arthritis in the rat van eden 1996 current. Hindpaw volume was measured by the mean of an hydroplethysmometer ugo basile, italy immediately before arthritis induction basal value and 3, 7, 11, 14, 17, 21 days thereafter, for both the injected. Effect of umbelliferone on adjuvantinduced arthritis in rats by mapknfkappab pathway liu ouyang, yang dan, zengwu shao, shuhua yang. Oct 26, 2015 pain in arthritis may be experienced in regions outside the affected joint, and hyperalgesia may even be widespread. Chemokine receptor expression in rat adjuvantinduced arthritis. Adjuvant arthritis aa of rats is a widely used experimental model because in many respects it mimics arthritis in humans. Evaluation of complete freunds adjuvantinduced arthritis. The present investigation was performed to evaluate the antiarthritic activity of butanol fraction of punica granatum linn. To study the antiinflammatory properties of celastrol in vivo, aia rats were treated daily with this drug after the disease had already become symptomatic. Rat adjuvant arthritis aia is an experimental model of polyarthritis that has been widely used for preclinical testing of numerous antiarthritic agents, which are either under preclinical or clinical investigation, or are currently used as therapeutics in this disease 24. A solution of carrageenan in saline injected subcutaneously in rats induces an acute swelling that becomes maximal 35 hours after the injection and subsides by 24 hours winter et al.
Adult male wistar rats were studied in an adjuvant arthritis aa model induced by the injection of freunds complete. Establishment of a rat adjuvant arthritisinterstitial. Rheumatoid arthritis ra is a chronic inflammatory disorder characterized by cellular infiltration and proliferation of synovium, leading to progressive destruction of the joints. Effect of galantamine on adjuvantinduced arthritis in rats. Pain in arthritis may be experienced in regions outside the affected joint, and hyperalgesia may even be widespread. Adjuvant arthritis aa was induced by intracutaneous injection of 0. Aqueous extract of the fruits of the plant piper longum was studied for their anti rheumatoid activity in freunds adjuvant induced arthritis rats with the dose of 200 and 400 mgkg p. Arthritis was induced by injection of complete freund s adjuvant cfa in rats. Deterioration of lipid and heamatological parameters in. Female lewis rats 100 gm were injected subcutaneously at the base of the tail with 300 l 5 mgml lyophilized mycobacterium butyricum difco, detroit, mi in sterile mineral oil on day 0. Experimental ra model, aia, created by the administration of freunds adjuvant has been used extensively in studying the. Rheumatoid arthritis ra is a chronic systemic inflammatory disease mediated by t cells. Protocol for induction of adjuvantinduced arthritis aia in.
Induction of rat adjuvantinduced arthritis aia and tissue sampling. The combination of natural products with modern medicine poses the possibility of potential. The aia model is a highly reproducible model of reactive arthritis in rats, that can also be used to study inflammatory pain in rheumatic diseases. Active disease developed 1112 days after injection reaching a peak at 1721 days. One of its major drawbacks is hepatotoxicity, resulting in poor compliance of therapy. Effect of luff aegyptica leaf extract on body weigh in alloxan induced diabetic rats. We investigated whether rats with antigeninduced arthritis aia exhibit peripheral changes in skin innervation remote from the inflamed joint. Oct 05, 2015 adjuvant arthritis was induced, as mentioned previously refaat et al. Dissatisfied arthritis patients are likely to seek the option of complementary and alternative medicine such as bee venom. Effect of umbelliferone on adjuvantinduced arthritis in rats by mapk. Effective treatment of rat adjuvantinduced arthritis by. The aim of the present study was to investigate the therapeutic efficacy of synthetic peptides hpr1, hpr2 and hpr3, derived from the sequence of 65kd mycobacterial heat shock protein hsp, in the treatment of ra using adjuvantinduced arthritis aa animal model.
Study of the inflammatory process induced by injection of. Modulation of th1th2 cytokines and inflammatory mediators by hydroxychavicol in adjuvant induced arthritic tissues. Jan 26, 2009 arthritis was induced by injection of complete freunds adjuvant cfa in rats. Adjuvantinduced arthritis model washington biotechnology. Although conventional f344 rats also develop aia, the arthritis is less severe and incidence is as low as 20%. At day 18th, an increase in scratching behavior and signs of hyperalgesia are clearly established as compared with the adjuvants vehicleinjected group. However, an understanding of the pathogenesis of inflammation in the tmj has been based on data from other synovial joints 18. Effects of peritoneal lavage with lidocaine on survival of rats with fecal peritonitis.
Adult male wistar rats were studied in an adjuvant arthritis aa model induced by the injection of freunds complete adjuvant fca. Effect of fty720, a novel immunosuppressant, on adjuvant. At day 18th, an increase in scratching behavior and signs of hyperalgesia are clearly established as compared with the adjuvant s vehicleinjected group. The adjuvantinduced arthritis aia model, one of the longest established animal models for testing arthritis treatments, is a reliable model with less welldefined mechanism than cia or pia.
Paw swelling and hyperalgesia of aa rats were measured at various times after cfa administration. Ganghwaljetongyeum ghjty has been used as a standard treatment for arthritis for approximately 15 years at the korean medicine hospital of dongshin university. Arthritis was induced by a single intradermal dose 0. Vaccination with endosomal unknown epitopes produces. This arthritis model, called adjuvantinduced arthritis aia, has previously been used as a model for rheumatoid arthritis ra. Ethanol extracts of ayurvedic formulations vatari guggulu, pathyadya churna and shatpuspadya churna were administered to. Full text effect of umbelliferone on adjuvantinduced arthritis in rats. Adjuvantinduced arthritis in the temporomandibular joint of.
This t cell dysimmunemediated disease is one of the most important pharmacological models of rheumatoid arthritis and is commonly used to select classic nonsteroidal antiinflammatory drugs nsaids 6. Otterness and moore, 1988, whereas a subcutaneous injection of complete freund s adjuvant in saline induces a more prolonged swelling that becomes maximal at 24 hrs and persists for at. Celastrol is able to suppress inflammation in wistar rat adjuvantinduced arthritis. Investigation of analgesic effect of pde 4 inhibitors on. Rats were maintained in a specific pathogenfree laboratory in the animal center of tongji medical college under controlled temperature 22c. Adjuvant arthritis aa is an induced form of subchronic arthritis. Freund s complete adjuvant contains heatkilled dead mycobacterium tuberculosis bacteria in liquid paraffin in the concentration of 10 mgml. Effect of elaeocarpus sphaericus in freund s complete adjuvant fca induced rheumatoid arthritis in albino rats. Brocco mc, paulo dn, baptista jf, ferrari ta, azevedo tc, silva al. Freunds adjuvant induced arthritis model was used to access the antiarthritic activity in albino rats 16. Cyclosporine a cya, 2,5 mgkgday, methotrexate mtx, 0,3 mgkg, 2 times a week, ribomunyl 25 mgkg 4 times a week. Jul 25, 2014 rheumatoid arthritis ra is a chronic systemic inflammatory disease mediated by t cells.
490 383 1504 1617 373 1544 1131 312 209 1531 68 495 1296 1418 586 557 351 697 871 250 225 572 1086 863 540 611 438 1513 1077 1152 1360 1444 1231 1295 522 595 726 1190 874 289 1427 495 702